Table 3 Primary antibodies
Antigen | dilution radio | antigen retrieval | source | Temperature of incubation of primary antibody | Time of incubation of primary antibody | Time of DAB | Time of staining with hematoxylin |
|---|---|---|---|---|---|---|---|
CD4 | 1:20 | citrate buffer solution pressure cooking (pH 6.0) 2 min | Rabbit | 37°C | 1 hr | 6 min | 5 min |
IL-17 | 1:100 | Tris-EDTA(pH 9.0) 15 min | Rabbit | 37°C | 1 hr | 8 min | 5 min |
Foxp3 | 1:100 | Tris-EDTA(pH9.0) 15 min | Rat | 37°C | 1 hr | 10 min | 2 min |
CD8 | 1:50 | Citrate buffer solution pressure cooking(pH 6.0) 2 min | Rabbit | 4°C | Overnight | 8 min | 3 min |
perforin | 1:10 | EDTA(pH 8.0) pressure cooking 2 min | Mouse | 4°C | Overnight | 8 min | 3 min |