Figure 2

MMTV SAg isoforms isolated from the cDNA from 18 different tissues of normal C57BL/6J mice. A. Phylogenetic tree (nucleotide sequence) of MMTV SAg isoforms. Eighteen sets of unique MMTV SAg isoforms, which were isolated from 18 different tissues of normal C57BL/6J mice, were analyzed for their phylogenetic relatedness. A number of branching units of SAg isoforms were formed in the phylogenetic tree. Unique SAg isoforms that were found in more than one tissue type are indicated using various shapes and shades. Four reference SAg sequences from Mtv-8, Mtv-9, Mtv-17, and Mtv-30 were included for this analysis. B. Phylogenetic tree (putative amino acid sequence) of SAg isoforms. Eighteen sets of unique SAg isoforms were phylogenetically evaluated and a phylogenetic tree with a number of unique branching units was formed. Unique SAg isoforms that were found in more than one tissue type are indicated using various shapes and shades. Four reference SAg sequences from Mtv-8, Mtv-9, Mtv-17, and Mtv-30 were included for this analysis. black circle (identical to Mtv-17); gray triangle (identical to Mtv-9); black diamond (identical to Mtv-8); gray circle, black triangle, gray box, and black box (identical sequences among different tissue types). cLu (lung cDNA), cOv (ovary cDNA), cUt (uterus cDNA), cTh (thymus cDNA), cSG (salivary gland cDNA), cILN (inguinal lymph node cDNA), cSI (small intestine cDNA), cMLN (mesenteric lymph node cDNA), cALN (axillary lymph node cDNA), cKd (kidney cDNA), cSk (skin cDNA), cSt (stomach cDNA), cSp (spleen cDNA), cAG (adrenal gland cDNA), cBM (bone marrow cDNA), cBr (brain cDNA), cLi (liver cDNA), cCn (colon cDNA), N (normal tissue)