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Figure 1 | BMC Immunology

Figure 1

From: Efficient activation of T cells by human monocyte-derived dendritic cells (HMDCs) pulsed with Coxiella burnetii outer membrane protein Com1 but not by HspB-pulsed HMDCs

Figure 1

SDS-PAGE and immunoblot analysis of recombinant proteins Com1 and HspB. A SDS-PAGE analysis of Com1 and HspB: lane M, protein molecular mass markers; lane 1, lysate of E. coli cells harboring plasmid; lane 2, lysate of E. coli cells harboring com1-recombined plasmid; lane 3, purified 41 kDa Com1 fused with 17 kDa tagged-peptide from pET32a expression plasmid; lane 4, lysate of E. coli cells harboring plasmid; lane 5, lysate of E. coli cells harboring hspB-recombined plasmid; lane 6, purified 54 kDa HspB. B Immunoblot analysis of proteins Com1 and HspB with sera from mice experimentally infected with C. burnetii. Lanes 1, 3, 5, and 7, Com1 immunoblot assay with mouse sera obtained at days 7, 14, 21, and 28 postinfection, respectively, with C. burnetii. Lanes 2, 4, 6, and 8, HspB immunoblot assay with mouse sera obtained at days 7, 14, 21, and 28, respectively, postinfection with C. burnetii.

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BMC Immunology

ISSN: 1471-2172

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