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Figure 2 | BMC Immunology

Figure 2

From: Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda

Figure 2

Intensity plots from microarrays of zebrafish embryos treated by immersion in E. tarda , E. coli , or P. aeruginosa suspensions. Embryos were immersed at 25 hpf in E. tarda (a), E. coli (b) and Pseudomonas aeruginosa PAO1 (c) and PA14 (d) suspensions, or in clean egg water as a control. RNA for microarray analysis was isolated from pools of 20 embryos at 5 h post exposure (hpe). RNA samples from embryos exposed to bacterial suspensions and control embryos were hybridized against a common reference from all treatment groups. The intensity plots show comparisons of treatment versus control groups derived from re-ratio analysis against the common reference. Significantly (P < 0.0001) up-regulated genes are shown in red, down regulated genes are shown in green and remaining genes in blue.

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