Figure 2From: Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tardaIntensity plots from microarrays of zebrafish embryos treated by immersion in E. tarda , E. coli , or P. aeruginosa suspensions. Embryos were immersed at 25 hpf in E. tarda (a), E. coli (b) and Pseudomonas aeruginosa PAO1 (c) and PA14 (d) suspensions, or in clean egg water as a control. RNA for microarray analysis was isolated from pools of 20 embryos at 5 h post exposure (hpe). RNA samples from embryos exposed to bacterial suspensions and control embryos were hybridized against a common reference from all treatment groups. The intensity plots show comparisons of treatment versus control groups derived from re-ratio analysis against the common reference. Significantly (P < 0.0001) up-regulated genes are shown in red, down regulated genes are shown in green and remaining genes in blue.Back to article page