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Figure 6 | BMC Immunology

Figure 6

From: Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda

Figure 6

Marker gene expression in individual embryos in response to injection of E. tarda. Expression levels of cyp1a (a), irg1l (b), mmp9 (c) and il1b (d) were measured by qPCR in 4 single embryos at 4 h and 8 h after injection (hpi) of approximately 200 CFUs of E. tarda into the caudal vein of embryos at 28 hpf. Control embryos were injected with PBS. Relative induction levels are shown with the lowest expression level set at 1. Lines with * indicate a significant difference of P < 0.05. Lines with ** indicate a significant difference of P <0.01 (tested by two-way ANOVA analysis of log-transformed data with the Bonferroni method as post-hoc test).

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