Figure 4
Systemic chemokine profiles in NZB/W F1 mice immunized with phthalate-KLH. NZB/W F1 mice (N = 5) were immunized as described under Materials and Methods, and sera collected (after 2nd booster, and then the 3rd booster with the antigen phthalate-KLH alone) were diluted 1:5 and subjected to cytokine and chemokine antibody arrays. Each cytokine was measured in duplicate spots and displayed in the array template as shown. Densities of each spot from images shown in Figure 3 were semi-quantified using image J software [27] and expressed as relative units to positive controls provided with the array. The image in (A) represents chemokine profiles in sera of mice immunized 3 times with the antigen and adjuvants, and the image shown in (B) represents chemokine profiles of mice treated receiving a 3rd booster immunization with antigen alone.