Figure 5
Systemic cytokines profiles of NZB/W F1 mice immunized with phthalate-KLH. NZB/W F1 mice (N = 5) were immunized as described in materials and methods, and sera collected after 2nd, and 3rd booster immunizations were diluted 1:5 and subjected to cytokine and chemokine antibody arrays. Each cytokine is represented by duplicate spots in the array as shown in the array template. Densities of each spot from images shown in figure 3 were semi-quantified using image J software and expressed as relative units to positive controls provided in the array. The image in (A) represents cytokine profiles in sera of mice immunized 3 times with the antigen and adjuvants, and the image shown in (B) represents cytokine profiles of mice treated receiving a 3rd booster immunization with antigen alone.