A-C: Effect of NSAID on leukocyte migration: changes of migratory activity of isolated human lymphocytes and granulocytes were measured using 96-wells calcein AM assay. Ketoprofen (A), diclofenac (B) or SC-560 (C) showed a dose-dependent inhibition of PMNs and lymphocyte migration. D-F: Toxic effect of NSAID on PMNs: Leukocytes were incubated for 60 min with different concentrations of ketoprofen (D), diclofenac (E) and SC-560 (F). Cell number was measured using Calcein AM uptake. Only highest concentrations of ketoprofen (4 mM) and diclofenac (2.5-5 mM) induced significant reduction of the leukocyte number.