Figure 4

Lck-EFNB1^f/f progenitors reconstitute the spleen in irradiated recipients. A. 2 ×10⁶ T-cell depleted bone marrow cells from Lck-Cre-EFNB1^f/f (top panel) or control EFNB1^f/f mice (bottom panel) were transferred to lethally irradiated C57BL/6 × SJL F1 recipients, followed by analysis 8 to 10 weeks later. Percentage of CD45.2⁺ cells (derived from Lck-Cre-EFNB1^f/f or EFNB1^f/f bone marrow cells), and CD45.1⁺/CD45.2⁺ cells (derived from residue cells of the recipients) is indicated. B. 1 × 10⁶ T-cell depleted Lck-Cre-EFNB1^f/f and EFNB1^f/f bone marrow cells (CD45.2⁺) were mixed with T-cell depleted bone marrow cells from B6.SJL competitor at 1:1 ratio, and transplanted to lethally irradiated C57BL/6 × SJL F1 recipients. After 8 to 10 weeks, cells from spleen were analyzed for CD45.2 and CD45.1 staining. Percentage of CD45.2⁺ cells (derived from Lck-Cre-EFNB1^f/f or EFNB1^f/f bone marrow cells), CD45.1⁺ cells (derived from competing B6.SJL bone marrow cells), and CD45.1⁺/CD45.2⁺ cells (derived from residue cells of the recipients) is indicated. In the WBI-BMTx models as described in Figure 4A and B, B220⁺ B cells and CD3⁺ T cells among CD45.2⁺ cells (derived from Lck-Cre-EFNB1^f/f or EFNB1^f/f bone marrow cells) in the spleen were determined by flow cytometry, and their percentage is shown.