Figure 1

Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220⁺ CD1d⁺ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1⁺ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d⁺ B220⁺ B cells relative to total B220⁺ B cells outside (MZ) and inside (FO) the Moma-1⁺ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.