Figure 4

Proliferative response of FOB or MZB cells from B6 or B6.TC mice. Proliferation after 3 d in culture with LPS (A), chromatin ICs (B), protein ICs (C), anti-CD40 (D), or activated CD4 T cells (E). Proliferation was assessed by CFSE dilution as the percentage of B220⁺ cells in the last two cell division cycles (CSFE-low). Means and SEM, Student t test comparisons: *: p < 0.05; **: p < 0.01, ***: p < 0.001. For A-D, representative histograms indicating the CFSE-low gate for B220-gated cells are shown for each stimulatory condition, with filled gray histograms corresponding to B6 FOB, thin black lines to B6.TC FOB, gray lines to B6 MZB and thick black lines to B6.TC MZB cells. F. Representative histogram showing surface CD154 expression on B6.TC (black) and B6 (gray) CD4⁺ T cells without (dotted lines) or with PMA/ionomycin stimulation (solid lines). CD154 median fluorescence intensity (MFI) is indicated for activated cells gated by the horizontal markers.