Skip to main content
Figure 7 | BMC Immunology

Figure 7

From: Selectively enhanced expression of prophenoloxidase activating enzyme 1 (PPAE1) at a bacteria clearance site in the white shrimp, Litopenaeus vannamei

Figure 7

Gene silencing of the prophenoloxidase-activating enzyme from white shrimp Litopenaeus vannamei (lvPPAE1) and related bioassays. (A) RNAi of lvPPAE1 using gene-specific dsRNA analyzed by qRT-PCR and normalized to β-actin. Five samples were mixed and analyzed 12 and 24 h after the first dsRNA injection and another 24 h after the second injection. (B) Phenoloxidase activity assay after gene silencing, analyzed by analysis of variance (ANOVA) followed by Duncan's multiple range test. Five individuals were taken as replicates. Bars with different letters are significantly different (P < 0.05). (C) Mortality of shrimps due to Vibrio harveyi after gene silencing. The experiment was conducted three times.*Occasional mortality occurred after the first lvPPAE1 dsRNA infection.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us