Figure 3

DNA recognition and signal transduction. RT-PCR analysis of type-I-interferon expression in HaCaT cells and HKC after preincubation with 10 μM of NFκB-, Erk2/MAPKK-, JAK/STAT-, JNK-, p38 MAPK- and PI3K-inhibitors (Sigma, Steinheim, Germany) for 1 h and stimulation with 5 μg S. cerevisiae DNA/ml medium for 6 h (HKC) and 15 h (HaCaT cells). The results were normalised to 18S ribosomal RNA (ratio: target gene/housekeeping gene) and compared to non-treated samples (vehicle control) (* = p < 0.05; ** = p < 0.005 to positive control (SC-DNA transfected, non-inhibited); # = p < 0.05; ## = p < 0.005 to vehicle control).