Figure 2

Determination of the roles of IP-10 protein in the activation of HCV replication. (A) Huh7.5.1 cells were infected with FL-J6/JFH-5'C19Rluc2Aubi virus for 24 h, and then incubated with PBS, IP-10 (1 μg/ml), or heat-inactivated IP-10 (HI-IP-10) (1 μg/ml) in 24-well plates for different times, as indicated. Treated cells were harvested and analyzed for luciferase activity. (B) Huh7.5.1 cells were infected with JFH1 virus, and then incubated with PBS, IP-10, or HI-IP-10 for 9 days. Culture supernatants were harvested and HCV copy numbers were determined by real-time PCR (RT-PCR). (C) Huh7.5.1 cells were infected with JFH1 virus, and then incubated with PBS, IP-10, or HI-IP-10 for 9 days. Cells were harvested and analyzed for HCV core protein expression by Western blot. β-Actin expression was measured as a loading control. (D) Huh7.5.1 cells were infected with FL-J6/JFH-5'C19Rluc2Aubi virus and then incubated with PBS, 1 μg/ml MSIgG, or 1 μg/ml anti-IP-10 neutralizing antibody for 144 h. Cells were harvested at different times as indicated and luciferase activity was assayed.