Figure 2

Expression of CD80, CD86 and CD40 on differentially primed BMDC after secondary CD40 ligation. Wildtype BMDC were stimulated with B. vulgatus mpk (MOI 1) or E. coli mpk (MOI 1) and LPS at low (1 ng/ml) or high concentration (1 μg/ml), respectively, for 24 hours. Unstimulated DCs (PBS) served as a control. Figure2A and 2B: The pre-treated DC were washed twice and were re-stimulated with an agonistic anti-CD40 mAb (5 μg/ml) for 48 hours. As a control, DCs were further incubated in medium only (mock) or in the presence of the isotype control. The expression levels of CD80 (A) and CD86 (B) were measured by FACS analysis. Figure2C: The pre-treated DC were washed twice and were re-stimulated with CD40L by co-culture with J558L/CD40L transfectants or were cultured in medium only as a control (− J558L/CD40L). After 48 hours, the expression of CD40 was determined by FACS analysis. Each histogram is representative of three independent experiments. The data are mean values of the percentages of the positive cell populations determined in these three experiments, ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05 compared to the respective control (PBS).