Figure 3

Phosphorylation of p38 MAPK upon secondary CD40 ligation in immature, semimature and mature DC. Wildtype BMDC were stimulated with B. vulgatus mpk or LPS 1 ng/ml (LPS^low) to generate semimature DC and E. coli mpk or LPS 1 μg/ml (LPS^high), respectively, to generate mature DC. Immature DC were maintained by incubation in the absence of further stimuli (PBS). After 24 hours, DC were re-stimulated with anti-CD40 mAb (1 μg/ml) for 15 minutes or treated with the isotype control (Isotype). The expression of pp38 was analyzed by Western blotting. As loading control, β-actin expression was determined. The results shown are representative of three independent experiments.