Biodistribution and elimination kinetics of systemic Stx2 by the Stx2A and Stx2B subunit-specific human monoclonal antibodies in mice

Table 1 Distribution of ¹²⁵ I-Stx2 in the kidney and liver following 3 h, 1 day and 2 days of ¹²⁵ I-Stx2 injection ¹

Sampling time	Tissue	PBS	5C12	5H8
3 h	Kidney	41±24.1^a	11±3.4^b	8±2.6^b
3 h	Liver	5±0.4^a	23±4.8^b	25±4.9^b
Day 1	Kidney	43±29.1^a	9±4.0 ^b	11±19.1^b
Day 1	Liver	4±1.9^a	10±1.8^b	8±1.9^b
Day 2	Kidney	13±1.7^a	4±1.2^b	1±0.^c
Day 2	Liver	1±0.1^a	3±0.6^b	2±0.1^c

¹Distribution of ¹²⁵I-Stx2 in the kidney and the liver following 3 h, 1 day and 2 days of ¹²⁵I-Stx2 injection. Mice were given intraperitoneally PBS or 30 μg of HuMAbs 5C12 or 5H8 followed 4 hours later with intravenous administration of 100 ng of ¹²⁵I-Stx2. Distribution of ¹²⁵I-Stx2 in the kidney and the liver was determined following 3 h, 1 day and 2 days of ¹²⁵I-Stx2 injection, and expressed as “% injected dose (ID)/g tissue”.
^a-c Values with different letters (superscripts ‘a’, ‘b’ and ‘c’) within a tissue indicate differences are statistically significant (p < 0.05) among those treatment groups. Values with same letter superscript within a tissue indicate differences are not statistically significant among those treatment groups.

ISSN: 1471-2172