Figure 2
From: A novel splice variant of folate receptor 4 predominantly expressed in regulatory T cells
Expression of the novel FR4 D3 variant in splenic T cell sub populations. (A) Isolation and purity (%) of splenic T cell subpopulations. (B) Purity of Foxp3-positive cells in isolated CD4+CD25+ T cells. (C) FR4D3 protein expression in particular T cell subsets was detected by Western blotting assay using anti-mouse FR4 mAb . Here, 10 μg of total cell extracts was treated with N-glycosidase before blotting. β-actin protein was detected as an internal control.