Pulmonary γδ T cells are increased following S. aureus infection. C57BL/6 mice were intranasally infected with SA 75 strain or PBS as mocked infection and sacrificed at different time post infection. (A) Pulmonary leukocytes were isolated and TCRαβ– TCRγδ+ cells were detected by flow cytometry. Cells were gated on CD3+ cells. Representative flow cytometry dot-plots were shown. Numbers represent the percentage of γδ T cells. (B) Absolute numbers of γ δ T cells in the total lung at various time points following S.aureus challenge were shown. (C) The expression of Vγ1 and Vγ4 chains on gated CD3+ TCRαβ- TCRγδ+ lung cells were analyzed by flow cytometry. Numbers indicate percentages of Vγ1+ or Vγ4+ γδ T cells. Representative flow cytometry dot-plots were shown. (D) Absolute numbers of Vγ1+ or Vγ4+ γδ T cells in naive and 6 h post-infection lungs. Bars represent mean ± SEM of 3–6 mice/time from three independent experiments. ** p < 0.01 versus naive control.