Figure 4From: Micrurus snake venoms activate human complement system and generate anaphylatoxinsProteolytic action of the Micrurus spp venoms on human purified C3. [A] Samples of purified component C3 (1 μg) were incubated with 2 μg of Micrurus spp venoms or PBS for 30 min at 37°C. Inhibition assays were performed by including 10 mM of 1,10 phenanthroline [B] or PMSF [C] in the mixtures. Cleavage was visualized by western blot using anti-C3 serum.Back to article page