ERK1/2 phosphorylation levels in Monocyte-Derived Macrophages from ascites of patients with HCV-C or ALC-C. A) Representative immunoblots for p-ERK1/2 of peritoneal M-DM from patients with HCV-C (n = 4) or ALC-C (n = 12) at baseline and following LPS treatment. β-actin was measured as loading control. B) Immunoblots were quantified and the average ± SEM ratios of phosphorylation are represented as bar graphs referred to β-actin. Wilcoxon signed-rank test: # P < 0.05, ###P < 0.001, between control (normalized as 1) and treatments. Mann–Whitney U test: *P < 0.05, between ALC-C and HCV-C.