Figure 1

The production of IFN-γ induced by peptide N50. BALB/c mice were immunized (i.m.) by SARS CoV S DNA. One to two weeks after the final boost immunization, splenocytes were prepared and stimulated with peptide N50. (A) After 14–18 h, the number of IFN-γ producing cells was detected by ELISPOT. Representation of ELISPOT results was shown. (B) After 4–5 h, FACS was performed to determine the percentages of IFN-γ⁺ cells in both CD4⁺ and CD8⁺ T cell population. Representation of FACS results was shown. Numbers at the corner in each sample represent the percentage of positive cells. (C) ELISPOT results. (D) After 72 h, supernatants were collected and levels of IFN-γ were detected by ELISA. (E) FACS results. Each open symbol represents mean value of an independent experiment (n =6). Cross bar represents the mean result. “0” represent non-peptide control.