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Figure 4 | BMC Immunology

Figure 4

From: Adoptive transfer of IL-4Rα+ macrophages is sufficient to enhance eosinophilic inflammation in a mouse model of allergic lung inflammation

Figure 4

Transfer of IL-4Rα+/+ BMM enhances the TH2-driven, OVA-induced eosinophilic inflammation in the lungs and airways of mice. BMM were prepared from IL-4Rα+/+ or IL-4Rα-/- mice as described above. BMM (5 × 106) were transfered by IP injection to IL-4RαxRAG2-/- mice on day -1. On day 0 mice were injected with PBS alone or with TH2 cells derived from D011.10 mice as indicated (1 × 107). The mice were immunized with OVA/alum on day 1 as indicated, followed by boost and challenge as described in Figure 2. Lung sections from OVA/alum primed mice were prepared and stained with H&E. A. Several digital images (A-F) from a representative mouse from each group (n = 4) are shown at 10 and 20X. B. The slides from individual mice were evaluated for the degree of pathology (0-3) as described in Materials and Methods. The mean score for each group is represented in the form of bar graphs ± SEM. In most cases the SEM was too small to show on the graph. Significant differences between the two groups noted were determined using the Student's T-test. ** (p < 0.01) C. BMM were prepared as described above and transfered by IP injection to IL-4RαxRAG2-/- mice on day -1. On day 0 all mice were injected with TH2 cells derived from D011.10 mice (1 × 107). The mice were immunized with alum or OVA/alum on day 1 as indicated, followed by boost and challenge as described in Figure 2. The BAL from individual mice (n = 3-4) was evaluated by differential counting after cytospin. Total numbers of cells present in the BAL are represented in the form of bar graphs ± SEM (T, total cells; M, macrophages; E, eosinophils; L, lymphocytes; and P, polymorphonuclear leukocytes). ** Significant differences between the two groups noted were determined using the Student's T-test (p < 0.01). D. The percentage of eosinophils in the BAL isolated from the individual mice is shown with the mean of the values represented by the bar. Significant differences between groups were determined using the Student's T-test. * (p < 0.05) These results are representative of 3 independent experiments.

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