Figure 5

Specific CD4⁺T cell responses in mice immunized with peptide L1 and L2. (A) CD4⁺ T cells immunized with Lpp20-derived peptides were tested in proliferative responses to peptide L1 and L2 (1.25 μg/ml), rLpp20 (15 μg/ml) and control medium. CD4+ T cells isolated from mice treated with PBS in IFA were served as negative controls. Response to the antigen was expressed as mean SI of three independent experiments ± S.D. (*) SI was higher than 2 at the 95% confidence level. SI ≥ 2 was considered as positive. (B) FACS analyses were performed to determine the relative percentage of CD4⁺ CD3⁺ T cells immunized with peptide L1 and L2 respectively. Control group was immunized with PBS emulsified in IFA. All data were reported as means ± SD of three experiments. *p < 0.05 vs. control.