Figure 3

Chronic inflammatory microenvironment induces a shift of CD11b⁺cell populations. A. Proportion of splenic CD11b⁺ cell population in BALB/c control animals and animals inoculated 14 days earlier with pristane i.p. B. Proportion of different CD11b⁺ cell populations in spleen and granuloma inoculated 14 days earlier with pristane i.p. C. RT-PCR analysis of S100A9 RNA expression in sorted cells from spleen and granulomas from animals inoculated 14 days earlier with pristane i.p., as indicated. The threshold cycle number was determined and relative expression compared to the reference gene (β-actin) and the target gene, respectively. Statistical analysis was performed using Student’s t-test (*p < 0.05; **p < 0.01;***p < 0.001).