Figure 4

Arginase and iNOS expression are very similar in CD11b⁺cell subpopulations from tumors or chronic inflammatory microenvironments. Indicated CD11b⁺ cell populations were isolated by cell sorting from spleen of control naïve mice (A, D), or from spleen and tumor tissue of mice carrying EL4 tumor (B,E) or from spleen and granuloma tissue of mice carrying peritoneal granulomas (C,F). RNA was isolated from the sorted cells and qRT-PCR was performed to determine expression of Arginase I (Arg) or iNOS relative to β-actin expression, as indicated. qRT-PCR was performed in triplicate and data displayed as mean expression +/− SD. The same mRNA preparations were analyzed in A-C and D-F.