Figure 4

BATF regulates IL-17 production by primary iNKT cells. A Glycolipid-stimulated splenocytes of the indicated genotypes were analyzed by ELISA for secreted levels of IL-4 and IL-17A after 72 h. Graphed are data averaged from 6 mice per group (n = 6). Bars indicate standard error. *p values < 0.05. B iNKT cells were enriched from pooled PLN of the indicated genotypes, stimulated for 4 h, and NK1.1^- IL-17A⁺ cells quantified by flow cytometry. Representative plots gated on tetramer⁺ cells are shown on the left with the % of cells in each quadrant indicated. The mean and distribution of data from at least 5 mice per genotype are presented on the right (n ≥ 5). *p values < 0.05.