Figure 5

DN32.D3 hybridoma cells display features of iNKT-17 cells. A DN32.D3 cells were stimulated with plate-bound mouse CD1d dimers either loaded with aGalCer (+) or not (−) and after 24 h, RNA was prepared and analyzed by qRT-PCR for the indicated transcripts. Shown are the averages of 4 samples (n = 4). Bars indicate standard error. *p values < 0.05. B DN32.D3 cultures stimulated as in (A) for 24 h and RNA analyzed for Il17a and Ifnγ transcripts. The mean of 4 samples is presented (n = 4). Bars indicate standard error. *p < 0.05. C DN32.D3 cells were treated with siRNA for 24 h, washed and stimulated as in (B) for 2 h. RNA was isolated and analyzed for the indicated transcripts. Data shown are averaged from 5 samples (n = 5). Bars indicate standard error. *p values < 0.05.