Figure 1
From: Expression profiling of lymph node cells from deer mice infected with Andes virus
Algorithm for producing real-time primers for use in deer mouse PCR arrays. Lab mouse cDNA sequences were BLASTed against the deer mouse genome to produce virtual cDNAs of deer mouse genes. These sequences were then piped to Primer3 software to identify candidate real-time PCR primers. Once pathways of interest have been identified, new genes can subsequently be examined using the same algorithm.