Figure 2

IL-17 induces a decrease in both neurosphere volume and cell number. NSCs were cultured at 400 cells/ml in 96- or 12-well plates and treated with IL-17, TNF-α and IL-10 for 96 h, as described in Materials and Methods. (a) NSCs treated with different concentrations of IL-17; (b) Number of NSCs treated with different concentrations of IL-17; (c) NSCs treated with IL-17 (25 ng/ml), TNF-α (25 ng/ml) and IL-10 (50 ng/ml) alone; (d) Number of NSCs treated with IL-17 (25 ng/ml),TNF-α (25 ng/ml) and IL-10 (50 ng/ml) alone. Cell number and the number of living neurospheres were measured using an inverse microscope. Mean percentages were determined from five independent experiments. NS, not significant.