Figure 4

T cells stimulated with proTα- or proTα(100–109)-matured DCs are cytotoxic in the presence of HER-2(9 ₃₆₉ ). (A) Intracellular production of TNF-α (left) and surface expression of CD107a (right) in CD8+ T cells stimulated with DCs matured for 48 h with TNF-α (first row), proTα (middle row) or proTα(100–109) (bottom row), in the absence (−) or presence (+) of the HER-2/neu peptides. Numbers indicate percentages of positive cells on gated CD8+ T cells. Shown are data from one representative experiment of 5 performed. (B) T cells stimulated with TNF-α-, proTα- or proTα(100–109)-matured DCs, were used as effectors (E) against unloaded, HER-2(9₃₆₉)- and tyr(9₃₆₉)-loaded T2 targets (T). Where indicated, mAb to MHC class I molecules was added throughout the culture period at a final concentration of 5 μg/mL. In all assays the E:T ratio was 10:1. Data represent mean % cytotoxicity ± SD from 2–5 donors.