Figure 4

Human macrophages are the dominant mononuclear cells in the lesions that promote BCG growth. A. Lung sections prepared from NSG-A2-BLT mice infected with BCG as described in Figure 1 were stained with FITC-labeled anti-human CD68 and FITC-labeled anti-mouse F4/80 (200×, scale bars = 50 μm) (A). Lung sections were stained with anti-PPD antibody followed by secondary antibody labeled with Alexa 555. DAPI was used for counter staining (200×, scale bars = 50 μm) (B). Images are representative of tissues from four animals.