Figure 4

1 α , 25-dihydroxyvitamin D3 increases the ratio of regulatory to effector T cell phenotype. A Contour plots showing CD4⁺CD25⁺FoxP3^hi populations and CD4⁺CD25⁺Foxp3^low T cells after 7 days culture with depigmented-polymerised (Depig-pol) or native grass pollen extracts with and without 1α, 25-dihydroxyvitamin D3 (10^-7 M). B Ratios of Foxp3^hi to Foxp3^low T cells in cultures stimulated for 7 days with HDM or grass pollen with and without 1α, 25-dihydroxyvitamin D3. N = 5, analysis by paired t-test.