IFN-γ does not change histamine content and FcϵRI-dependent mediator release of MCs. MCs were cultured for 14 days in medium supplemented with SCF (50 ng/ml), with SCF and IFN-γ (100 ng/ml), with SCF and IL-4 (2 ng/ml), or with all three cytokines. (A) Total cellular histamine content of MC measured after cell lysis (n = 7). (B) Histamine (n = 5) and (C) LTC4 (n = 4) release after stimulation of MCs by FcϵRI crosslinking for 30 min using mAb 22E7 (100 ng/ml). All data are shown as mean ± SEM; n.s. = non significant.