Binding of IgG subtypes on intestinal MCs. MCs were cultured in the presence of SCF with or without addition of IFN-γ (100 ng/ml) for the last 3 days. Then, MCs were incubated with IgG1-4 (500 μg/ml), respectively, or buffer control for 60 min. Binding of IgGs was analyzed by FACS with mAbs specific for the respective IgG subtype (dark gray peak). Isotype control (bright gray peak). One experiment out of three is shown.