Evaluating the impact of amplification. A: Gene-specific amplification determined by qPCR. Scatter plot comparing dCq for a gene between two cell lines for non-amplified samples with dCq for the same gene and cell lines after amplification. Pearson’s correlation coefficient is r = 0.917. Genes included PPIA, TBP, GAPDH, IRF4, PRDM1, XBP1, WHSC1 and MGST1. B: Comparing NuGEN protocol to standard protocol. Scatter plot comparing the Exon expression values generated by the NuGEN protocol to expression values generated by the Ambion protocol. Genes included are PPIA, TBP, GAPDH, IRF4, PRDM1, XBP1, WHSC1, and MGST1. The Pearson’s correlation coefficient is r = 0.950.