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Figure 5 | BMC Immunology

Figure 5

From: Vitamin D receptor expression controls proliferation of naïve CD8+ T cells and development of CD8 mediated gastrointestinal inflammation

Figure 5

Proliferation of CD8+T cells in vitro . (A) Histograms show CFSE dilution induced following in vitro proliferation of sorted WT and VDR KO CD8+ T cells in response to CD3/CD28 stimulation. The shaded histograms show the CFSE stained T cells prior to stimulation with CD3/CD28, and open histograms are CFSE dilution following stimulation. Values are mean ± SEM of the frequency of T cells that have divided and diluted the CFSE label. (B) The CFSE labeled T cells in panel A were evaluated for CD8 subsets with or without (unstim) CD3/CD28 stimulation. Naïve CD8+ T cells are CD62LhighCD44low, and activated CD8+ T cells are CD62LlowCD44high. The shaded histograms are the CFSE staining at the beginning of the culture, and open histograms are CFSE dilution. Values are the mean of two independent experiments ± SEM. ANOVA, *P < 0.05.

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BMC Immunology

ISSN: 1471-2172

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