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Table 1 Mean 3-nitrotyrosine levels in plasma from mussels (n = 20) before and after PMA-stimulation. 3-nitrotyrosine contents of plasma proteins were determined in triplicate by C-ELISA as described in the Material and Methods and expressed as BSANT equivalents. "unstimulated": plasma from unstimulated haemolymph, "+ PMA": plasma 30 min after addition of 10 μL PMA (1 mg mL-1) to haemolymph, "PMA + L-NIO": plasma after 5 min incubation of haemolymph with L-NIO and 30 min with PMA.

From: In vitro production of peroxynitrite by haemocytes from marine bivalves: C-ELISA determination of 3-nitrotyrosine level in plasma proteins from Mytilus galloprovincialis and Crassostrea gigas

Unstimulated

+ PMA

+ PMA + L-NIO

0.037 ± 0.025 (n = 20)

0.118 ± 0.024 (n = 20)

0.082 ± 0.024 (n = 20)