Table 2 Mean 3-nitrotyrosine levels in plasma from mussels (n = 20) and oysters (n = 18) before and after phagocytosis of zymosan particles. 3-nitrotyrosine contents of plasma proteins were determined in triplicate by C-ELISA as described in the Material and Methods and expressed as BSANT equivalents. "unstimulated": plasma from unstimulated haemolymph, "zymosan": plasma 30 min after addition of 50 μL zymosan (40 mg mL-1) to haemolymph, "zymosan + DPI": plasma after 5 min incubation of haemolymph with DPI and 30 min with zymosan particles.
Unstimulated | Zymosan | Zymosan + DPI | |
|---|---|---|---|
Mussels | 0.037 ± 0.025 (n = 20) | 0.214 ± 0.023 (n = 20) | 0.117 ± 0.036 (n = 20) |
Oysters | 0.023 ± 0.017 (n = 18) | 0.176 ± 0.022 (n = 18) | 0.127 ± 0.037 (n = 18) |