Skip to main content

Table 1 Characteristics of different parallel plate flow chambers

From: Improvements to parallel plate flow chambers to reduce reagent and cellular requirements

  

Classic

GlycoTech

 
  

Single-Pass

Single-Pass

Re-circulation

  

Chamber

Chamber

Chamber

Cells Needed For

   

Shear Curve

   
 

0.062" I.D.

1 × 107 cells

2.4 × 106 cells

N/A

  

(5,000 ul)

(1,175 ul)

 
 

0.02" I.D.

9.9 × 106 cells

2 × 106 cells

N/A

  

(4,950 ul)

(1,000 ul)

 

Leucocytes Per

   

Constant Shear

   

Experiment *

   
 

0.062" I.D.

1.2 × 107 cells

2.2 × 106 cells

4.1 × 106 cells

  

(6,000 ul)

(1,075 ul)

(2,070 ul)

 

0.02" I.D.

9.9 × 106 cells

1.8 × 106 cells

1.7 × 106 cells

  

(4,950 ul)

(900 ul)

(850 ul)

Dead Volume

   
 

0.062" I.D.

1,500 ul

225 ul

2,070 ul

 

0.04" I.D.

820 ul

110 ul

1,290 ul

 

0.02" I.D.

450 ul

50 ul

850 ul

 

0.01" I.D.

360 ul

35 ul

740 ul

Number Of Constant

   

Shear Experiments

   

Per Cell

   

Preparation *

   

Neutrophil

2

15

23

 

Eosinophil

0

4

5

 

Basophil

0

2

4

Reduction In Cell/

   

mAb/Reagent Use

   
 

0.062" I.D.

0%

82%

66%

 

0.02" I.D.

0%

82%

83%

  1. * Number of experimental conditions calculated assuming 0.02" I.D. for the improved chamber and 0.062" I.D. for the classic chamber. I.D., internal diameter; N/A, not applicable, see text for details. Approximate cell number per preparation is 3 × 107 neutrophils, 2 × 106 eosinophils, and 6 × 105 basophils per preparation. Dead volume refers to the entire fluid volume external to the flow path.
Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us