Decreased inducibility of TNF mRNA in macrophages incubated with ac-LDL. Macrophages were cultured for 48 h in the presence of 5% LPDS ± 50 μg/mL ac-LDL. Half of the dishes were then stimulated with TNF (10 ng/mL) for 2 h. TNF mRNA was analysed by Northern blotting, using β-actin as loading control. Lanes: 1, Control; 2, TNF; 3, ac-LDL; 4, ac-LDL + TNF. Data are representative of three separate experiments.