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Figure 5 | BMC Immunology

Figure 5

From: Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand

Figure 5

Effects of IL-1 family members A, IL-12 secretion is modulated by IL-1α or IL-1β, but not by IL-18. Human monocyte-derived DC were treated with CD40L (1 μg/ml) for 24 hours in the presence of increased concentrations of IL-1α, IL-1β or IL-18 (0 to 100 or 500 ng/ml). Secreted IL-12 heterodimer was measured in supernatants by ELISA. B, Blocking IL-1Ra increases CD40L-induced secretion of IL-12 heterodimer. DC were activated as indicated in the presence of either anti-IL-1Ra neutralizing Ab or normal goat IgG for 24 hours. Supernatants were harvested and analyzed for IL-12 heterodimer by ELISA. The average secretion in duplicate wells was calculated as pg/1000 cells. Results are representative of three experiments. * indicates statistical significance of anti-IL-1Ra Ab treatment.

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BMC Immunology

ISSN: 1471-2172

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