Figure 1

FL promotes outgrowth of immature monocytic cells from mouse bone marrow in vitro (A) FL expanded non-adherent cells were counted after different culture durations. Data are expressed as % of initial cell number and represent mean ± SEM of five independent experiments (3 experiments for day 11). (B) FL-dose-dependent proliferation was assessed by using the MTT assay after 6 days of culture in 96-well plates. Data are mean ± SEM of three independent experiments. (C) Tracking division of day 6-FL cells (left panel) and day 8-FL cells (right panel). Cells were stained with CFSE then used for reseeding original culture flasks. Division number is indicated based on sequential halving of CFSE fluorescence. Data are representative of three independent experiments.