Figure 2

Guinea pig T cell epitopes in B. lentus subtilisin and BPN' Y217L. Hartley strain guinea pigs were immunized with either B. lentus subtilisin or BPN' Y217L. Splenocytes from immunized Hartley Guinea pigs were incubated in vitro with 15 mer peptides describing the sequence of the immunizing protein as described in the material and methods section. An epitope region was assigned if the T cell proliferation to a peptide reached an average SI of 3.0. Two major T cell epitopes were found in B. lentus subtilisin (underlined). No T cell epitopes meeting our criteria were found in BPN' Y217L. The first B. lentus subtilisin epitope has a very similar region in BPN' Y217L (in brackets) which contains the single amino acid change of I 72 V.