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Table 1 Pairs of synthetic primers used in RT-PCR to amplify the different molecular systems studied.

From: Auto-protective redox buffering systems in stimulated macrophages

Gene studied Sens sequence Anti-sens sequence size (pb)
HPRT 5'TGG AAT CCT GTG GCA TCC ATG AAA C 3' 5' TAA AAC GCA GCT CAG TAA CAG TCC G 3' 348
catalase 5' GCA GAT ACC TGT GAA CTG TC 3' 5' GTA GAA TGT CCG CAC CTG AG 3' 229
GCS 5' CCT TCT GGC ACA GCA CGT TG 3' 5' TAA GAC GGC ATC TCG CTC CT 3' 346
Gpx 5' CCT CAA GTA CGT CCG ACC TG 3' 5' CAA TGT CGT TGC GGC ACA CC 3' 197
GR 5' AGC CGC CTG AAC ACC ATC TA 3' 5' CCG TCT GAA TGC CCA CTT TA 3' 601
NOS(2) 5' ACG CTT CAC TTC CAA TGC AAC 3' 5' TGA GGG CTG ACA CAA GGC CTC 3' 511
SOD(Cu/Zn) 5' AAG GCC GTG TGC GTG CTG AA 3' 5' CAG GTC TCC AAC ATG CCT CT 3' 246
SOD(Mn) 5' GCA CAT TAA CGC GCA GAT CA 3' 5' AGC CTC CAG CAA CTC TCC TT 3' 241
Trx 5' CCC TTC TTC CAT TCC CTC TG 3' 5' AAC TCC CCC ACC TTT TGA CC 3' 149
TR 5' TCC TCT TTT TCT ACC CAC TG 3' 5' GTA TTC CTT GCT GTC ATC CA 3' 464