Severe anaphylactic reactions to glutamic acid decarboxylase (GAD) self peptides in NOD mice that spontaneously develop autoimmune type 1 diabetes mellitus

Table 1

Immunization^a	Challenge^b	Number of Mice with Anaphylaxis
		Temperature Decrease ≥ 1 °C	Death (all mice)	Death (in mice with anaphylaxis)
G7 peptides	G7 peptides	14/14 (100%) *	12/14 (86%)**	12/14 (86%)***
PD peptides	PD peptides	3/7 (43%) †	1/7 (14%)	1/3 (33%)
HEL/OVA	HEL/OVA	5/12 (42%) ‡	4/12 (33%) ‡‡	4/5 (80%) ‡‡‡
Saline	Saline	0/13 (0%)	0/13 (0%)	-
None (no injection)	None (no injection)	0/9 (0%)	0/9 (0%)	-
No injection	G7 peptides	0/9 (0%)	0/9 (0%)	-
Passive Systemic IgE-induced Anaphylaxis
IgE anti-DNP	DNP-HSA	10/10 (100%)#	0/10 (0%)	0/10 (0%)

a Immunizations in IFA b Challenge in 0.9% NaCl (saline) * P = 0.0058 vs. PD challenged, P = 0.0012 vs. HEL/OVA challenged, P < 0.0001 vs. G7 challenged non-immunized mice, saline challenged or "none" groups; † P = 0.031 vs saline challenged; ‡ P < 0.05 vs G7 challenged non-immunized mice, saline challenged or "none" groups; # P = 0.0147 vs PD challenged, P = 0.0053 vs HEL/OVA, P < 0.0001 vs G7 challenged non-immunized mice, saline challenged or "none" groups.; ** P = 0.0138 vs. HEL/OVA challenged, P < 0.0001 vs DNP-HSA challenged; ‡‡ P = 0.048 vs saline challenged; *** P < 0.0001 vs DNP-HSA challenged; ‡‡‡ P = 0.0037 vs DNP-HSA challenged; all other comparisons are P > 0.05 (Fisher exact test).

ISSN: 1471-2172