Figure 2

LFA-3 and MHC staining in cells infected by CMV wild type and CMV lacking UL16. Hs27 fibroblasts were either mock-infected (filled histograms) or infected with the laboratory strain of CMV AD169 (dotted line) and with the same strain of virus lacking UL16, ΔUL16 (solid line). Cells were stained for LFA-3 (A) and for MHC class I (B) and analysed on flow cytometry. LFA-3 was up-regulated in CMV infected cells to the same level, whether UL16 was present or not, compared to mock infected cells. At the same time, MHC class I became down-regulated in both CMV wild type and ΔUL16CMV infected cells in a comparable manner. Data are representative of several experiments and results obtained using both human fibroblast cell lines Hs27 and HFFF2 were comparable.