Western immunoblot from SDS-PAGE with gradient 4–15% acrylamide-bis. Paths 1–3: W-L cock serum diluted 1:4, 1:2 and 1:1, respectively. 4: prestained MW standards high range. 5: nothing. 6: prestained MW standards low range (the three asterisks point out those prestained MW that are weak). 7–8: W-L hen serum diluted 1:2 and 1:1, respectively. 9–10: P-R hen serum diluted 1:2 and 1:1, respectively. The stars point out each one of the four bands. The primary antibody was anti-ceE IgY adsorbed with sera from W-L hen and P-R hen, and the secondary antibody was adsorbed with liver extract from W-L hen. Four sAlloAg from W-L cock serum are present in the 53-hour-old chick embryo with circa 200, 70, 34, and 32 kDa. This adsorbed anti-ceE IgY does not detect any antigen in sera from W-L and P-R hens (paths 7–10).