Figure 7
From: Performance of plate-based cytokine flow cytometry with automated data analysis
Automated gating of HIV-1-seropositive CFC samples. (A) Correlation of automated gating to expert manual gating. Whole blood from 39 HIV-1-seropositive donors was stimulated using the antigens shown, then analyzed manually or with the automated template of Figure 6A. (B) Revised template designed for samples with very low numbers of CD4+ cells. A fixed region for CD3+CD4+ cells (R3) has been created and tethered to the automated region R2. (C) Improved performance of the revised template for calculating CD4+ responses from the dataset of (A). Note that the slope of the linear regression line for pp65 peptide mix and SEB stimulation is now closer to 1.