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Table 3 Tracking FITC-Subtilisin in an antigen uptake experiment with acid extractiona

From: Fluorescent derivatization of a protease antigen to track antigen uptake and processing in human cell lines

Cell Fraction

RFUs

Protein (μg)

% Recovered

Conjugate added

140,000

15555

100%

Washed cells

750

83

0.53

Cell extract

261

29

0.18

< 10 K fraction

26

2.9

0.018

  1. a5 × 108 8.1.6 cells were resuspended in 300 ml of complete media, mixed with 15.5 mg of fluorescein-subtilisin and incubated for at 37°C for 24 hr in a roller bottle.
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BMC Immunology

ISSN: 1471-2172

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